تصنيع جسيمات أكاسيد النحاس النانوية الخضراء المنتجة من نبات الحلفا وتقييم فعاليتها في تثبيط نمو خلايا (HCT-116) والنشاط المضاد للبكتيريا
DOI:
https://doi.org/10.54153/sjpas.2025.v7i1.829الكلمات المفتاحية:
Keywords: Imperata cylindrical, Copper oxide nanoparticles, Anticancer, HCT-116 ,Antibacterialالملخص
تبحث الدراسة الحالية لدراسة مضادات السرطان ومضادات البكتيريا مختبريًا لأكاسيد النحاس النانوية (CuO NPs) التي تم تصنيعها من نبات الحلفا Imperata cylindrical باستخدام اختبار MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide). وطريقة الانتشار القرصي تم تشخيص الجسيمات النانوية المصنعة باستخدام تقنيات تحليلية مختلفة مثل الأشعة فوق البنفسجية- المرئية، و الأشعة تحت الحمراء، وتحليلات الأشعة السينية المشتتة للطاقة، والمجهر الالكتروني الماسح، ومجهر القوى الذرية، وتحليل إمكانات زيتا. تمت دراسة كفاءة CuO NPs على خطوط خلايا سرطان القولون البشرية (HCT-116) وخلاياHFF) ) كخطوط خلايا طبيعية. تم اختيار تركيزات مختلفة من جسيمات أكسيد النحاس النانوية (10، 50، 100، 250،500) ميكروغرام / مل عند 24 ساعة. أظهرت النتائج أن تأثير الجسيمات النانوية يعتمد على التركيز. ومع زيادة تركيز الجسيمات النانوية تنخفض نسبة صلاحية الخلية. وجد أن تركيز جزيئات أكسيد النحاس النانوية عند 250 ميكروغرام/مل أعطى نسبة حيوية الخلية (30.08%) بعد 24 ساعة. كما أظهرت الجسيمات النانوية تأثيرات مضادة للبكتيريا ضد البكتيريا الموجبة والسالبة الغرام وبتراكيز مختلفة. بتركيز 1024 (ميكروجرام/مل)، أظهرت CuO NPs تأثيرًا قويًا مضادًا للبكتيريا ضد جميع أنواع البكتيريا الأربعة.
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