New Bioremediation by Lipase Purified from Ralstonia mannitolilytica for Petroleum Hydrocarbons
DOI:
https://doi.org/10.54153/sjpas.2024.v6i1.656Abstract
A growing issue of contaminated soil caused by oil derivers in Iraq, requires detection of new methods for bioremediation. The present work aims to purify of extracellular lipase enzyme that can eliminate oil derivers. Seven strains of lipolytic bacteria were collected from Al- Dora electricity station soil Baghdad/ Iraq that polluted with oil derivers. Ralstonia mannitolilytica considered higher ability in lipolytic enzymes production. Crude lipase activity gave (1.66 U/ml). The purification procedure resulted in 8.9 fold with a 23.2% final yield. The purity of lipase and molecular weight confirmed by SDS-PAGE. The efficiency of ten aliphatic hydrocarbon compounds in the soil contaminated with diesel decreases after treatment with purified enzyme (7.6 U/ml) for 24 hr. at natural environment to mimic the nature compared with control. The results concluded the degradation of aliphatic hydrocarbon compounds by lipase enzyme that purified from local Ralstonia mannitolilytica isolates was an efficient (biological) treatment. Although there are studies related to bioremediation used different bacterial isolates types and enzymes such as Pseudomonas sp. none of it used Ralstonia mannitolilytica isolate and lipase enzyme as bioremediation of oil spills.
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