Phenotypic and molecular diagnosis of Pseudomonas aeruginosa isolated from contaminated soil from some Iraqi governorate

Authors

  • Afrah Abdullah Jasim Department of Chemistry, College of Education, University of Samarra
  • Wael Mohammed Mahdi Department of Biotechnology, College of applied Sciences, University of Samarra
  • Omar Raheem Khalaf AL-Obaidi Department of Biology, College of Education, University of Samarra

DOI:

https://doi.org/10.54153/sjpas.2024.v6i1.638

Abstract

Twenty-five samples of contaminated soil with organic waste were collected from three iraqi governorates: Salah Al-Din Governorate, which included the city of Samarra, Baiji, and Dujail, Baghdad Governorate, which included Abu Ghraib, Baghdad Center, Mahmudiyah, and Kirkuk Governorate, which included Kirkuk, Hawija, and Daquq. The isolates were diagnosed phenotypically by the shape of the colony, its color and odor, and microscopically by its interaction with the gram stain. In addition,some biochemical tests were carried out, including(Catalase, Oxidase, IMVIC test, Biofilms production test, Kligler Iron test).The use of the Vitek 2 system to confirm the diagnosis of the isolates,as 9 isolates of P. aeruginosa bacteria were isolated, and the sensitivity test was conducted according to the Kirby-Bauer method (antibiotic disc diffusion) and using 8 types that included (TCC) Clavulanic, (PI) Piperacillin, )GEN) Gentamycin , (AK) Amikacin, Ceftazidime (CAZ), (IPM) Imipenem, (MPP) meropenem, Ciprofloxacin (CIP), as the results of the sensitivity test for soil isolates showed a different variation in their resistance and sensitivity. The results of the molecular diagnosis using PCR technique for specialized primers for the 16SrRNA gene and the phzABCDEFG operon showed that all bacterial isolates isolated from the soil belong to the type P.aeruginosa because they contain the specific gene 16SrRNA, while the results of the polymerase chain reaction of the isolates of P.aeruginosa bacteria showed the phzABCDEFG operon Not all isolates have the phzABCDEFG operon except for sample (S6)15.

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Published

2024-03-30

How to Cite

Abdullah Jasim, A., Mahdi, W. M., & AL-Obaidi, O. R. K. (2024). Phenotypic and molecular diagnosis of Pseudomonas aeruginosa isolated from contaminated soil from some Iraqi governorate. Samarra Journal of Pure and Applied Science, 6(1), 96–108. https://doi.org/10.54153/sjpas.2024.v6i1.638